EKSPLORASI POTENSI SENYAWA BIOAKTIF MAKROALGA LAUT Sargassum sp SEBAGAI ANTIBAKTERI ASAL PESISIR BARAT SELATAN (BARSELA) ACEH

Mohamad Gazali, Eri Safutra, Zulfadhli Zulfadhli, Neviaty P Zamani, Nurjanah Nurjanah

Abstract


West of Aceh coastal have highly marine biodiversity especially marine macroalga. Nowadays, marine macroalgae has not yet utilized optimally by local community as medicine and cosmestical raw material especially West-South of Aceh. One of the macroalgae species that possess bioactive compound that promising suffficiently is Sargassum sp. The aim of this research is to explore bioactive compound as antibacteria. In this study, activity of antibacteria of marine macroalga ethanol extract Sargassum sp was tested by using disc difussion method.  Bioactivity of exploration of such macroalga extract was expressed by minimum inhibitory concentration (MIC). Activity of antibacteria against negative gram bacteri Escherichia coli and positive gram bacteri Staphylococcus aureus  was discussed in this research. According to the result that minimum concentration Sargassum sp extract can inhibit bacteria is 1,25% whereas according to antibiotic standart of amphicilin for E. coli  is 10%, 5%, and 2,5% namely it have inhibition zone diameter 8,11 mm (strongly sensitive), 6,92 mm (sensitive) and 6,65 (sufficiently sensitive). Moreover, for S. aureus showed that  extract minimum concentration is 1,25% and 2,5% wth inhibition zone 6,0 mm whereas in concentration of 10% and 5% possess inhibition zone diameter 6,97 mm and 6,46 mm. It is showed the higher of concentration of Sargassum sp extract thus the higher also phenolic content  and tannin so, its extract can inhibit bacterial growth E. coli and S. aureus.

Full Text:

PDF

References


Angka S.L. dan Suhartono M.T. (2000). Pusat Kajian Sumber Daya Pesisir dan Lautan. Bogor: Institut Pertanian Bogor.

Anggadiredja, T.J., A. Zatnika, P. Heri, dan S. Istini, S. .(2009). Rumput Laut. Jakarta: Penerbit Penebar Swadaya.

Aslan,L.M., (1998). Budidaya Rumput Laut. Yogyakarta: Kanissius.

Arifuddin, Patong, R., dan Ahmad, A. (2001). Penelusuran Protein Bioaktif dalam Makro Alga sebagai Bahan Antibakteri dan Antijamur, Marina Chimica Acta.

Dawson, E.Y. (1946). Marine Botany and Introduction. Hollt, Rinehart and Winston, Inc. New York Chicago, San Fransisco, Toronto, London.

del Val AG, Platas G, Basilio A, Cabello A, Gorrochategui J, Suay I, Vicente F, Portillo E, del Río MJ, Reina GG, Peláez F. (2001). Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary Islands, Spain). Int Microbial 4:35–40

Indriani, H. dan Sumarsih, E. (1999). Budidaya Pengolahan dan Pemasaran Rumput Laut. Jakarta: PT Penebar Swadaya.

Putra, S.E., (2006). Alga Laut Sebagai Bio-target Industri, Situs Web Kimia Indonesia.

Kadi. (2008). Beberapa Catatan Kehadiran Marga Sargassum Di Perairan Indonesia. www.rumputlaut.org. 19/09/2017.

Luning. (1990). Seaweeds, Their Environment, Biogeography And Ecophysiology. New York: John Wiley and Sons.

Sastry and Rao. (1994). Antibacterial Substance From Marine Algae. Successive Extraction Using Benzene, Chloroform and Methanol. India: Department of Biochemistry, Institute of Medical Science, Banaras Hindu University.

Sumich. L. (1992). An Introduction To The Biology Of Marine Life. Wmc Brown. Dubuque. Lowa.

Sulistyowati, H. (2003). Struktur Komunitas Seaweed (rumput laut) di Pantai Pasir Putih Kabupaten Situbondo. Jurnal Ilmu Dasar. 4 (1): 58-61.

Tjitrosoepomo. (2005). Pengenalan Jenis Algae Hijau dalam Pengenalan Jenis-Jenis Rumput LautIndonesia. Jakarta: Puslitbang Oseanologi LIPI.

Zainuddin, E. N dan Malina, A, C. 2009. Skrining Rumput Laut Asal Sulawesi Selatan sebagai Antibiotik Melawan Bakteri Patogen pada Ikan. [Laporan Penelitian] Research Grant, Biaya IMHERE-DIKTI.


Refbacks

  • There are currently no refbacks.